Composite

Part:BBa_K782085:Design

Designed by: Fedja Pavlovec   Group: iGEM12_Slovenia   (2012-09-24)

10x[TALA] operator_minimal promoter_TALA:NLS:VP16_t2a_BFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 750
    Illegal BamHI site found at 720
    Illegal BamHI site found at 3262
    Illegal XhoI site found at 788
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 507
    Illegal AgeI site found at 12
    Illegal AgeI site found at 347
    Illegal AgeI site found at 372
    Illegal AgeI site found at 707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4207


Design Notes

design


Source

BFP was obtained from Evrogen.

References