Composite
Part:BBa_K782085:Design
Designed by: Fedja Pavlovec Group: iGEM12_Slovenia (2012-09-24)
10x[TALA] operator_minimal promoter_TALA:NLS:VP16_t2a_BFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 750
Illegal BamHI site found at 720
Illegal BamHI site found at 3262
Illegal XhoI site found at 788 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 147
Illegal NgoMIV site found at 507
Illegal AgeI site found at 12
Illegal AgeI site found at 347
Illegal AgeI site found at 372
Illegal AgeI site found at 707 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4207
Design Notes
design
Source
BFP was obtained from Evrogen.